Mass Spectrometry Core Facility
- Greg Barrett-Wilt, Ph.D.
425 Henry Mall Madison WI 53706
The University of Wisconsin Biotechnology Center Mass Spectrometry Core Facility has several mass spectrometers for the analysis of biomolecules, including proteins, peptides, oligonucleotides, oligosaccharides and other small organic and inorganic molecules. In addition to measuring the molecular weights of compounds, many of these instruments can perform MS/MS fragmentation data, so that the structure of a selected compound can be deduced from its fragments. This is particularly useful for peptide sequencing and post-translational modification mapping. The electrospray ionization (ESI) instruments are equipped with HPLC systems which can be used with reversed phase (RP) or hydrophilic-interaction (HILIC) chromatographies to better characterize complex mixtures by LC/MS and LC/MS/MS.
LC/MS/MS is extremely useful for quantitation of known compounds and for the rapid screening of mixtures for profiling and to identify components of interest. We also perform proteolytic digestions of proteins, which can be analyzed by HPLC, MS, LC/MS and LC/MS/MS for peptide mapping, peptide sequencing, and quantification.
The UWBC Mass Spectrometry Core Facility accepts samples from all Schools, Colleges, and Departments on the UW-Madison campus, across the University of Wisconsin System, academic institutions around the country, and commercial/industry clients around the world. Let us know how we can help you!