Contact Information
Mass Spectrometry / Proteomics
- Greg Barrett-Wilt, Ph.D.
Director
- Grzegorz (Greg) Sabat
Proteomics Specialist
Room 2250
Biotechnology Center
425 Henry Mall Madison WI 53706
Phone: 608/262-8732
FAX: 608/262-6748
Email: mass_spec@biotech.wisc.edu
The Mass Spectrometry Core Facility has established the Advanced Lipidomics Facility (ALF) through a collaboration with Dr. Judith Simcox and Dr. Dudley Lamming as a part of the UW-Madison Comprehensive Diabetes Center. This platform is based on a state-of-the-art Agilent 6546 QTOF mass spectrometer coupled with an Agilent 1290 Infinity II UHPLC system. The methods have been tested on several tissue types including plasma, intestine, skin, neuron, pancreas, liver, and adipose tissue.
Samples are submitted as frozen tissues, homogenized, extracted, and prepared for LC/MS/MS analysis. Data are collected in both positive and negative ion modes using C18-based chromatography. Data analysis is performed by the Mass Spectrometry Core Facility, with raw and processed outputs available for additional data analysis.
Please submit a completed submission form along with your sample, and provide an updated billing form when appropriate.
Mass Spectrometry Submission Form(Fillable PDF)
Blling Form(Fillable PDF)
| Product or Service | UW System Price | Non UW System Price |
|---|---|---|
| ESI MS | $55.00 | $91.52 |
| ESI MS/MS (triple quad) | $66.00 | $109.82 |
| GC/MS | $55.00 | $91.52 |
| GC/MS derivatization | $17.60 | $29.29 |
| In-gel Digest (trypsin) | $91.30 | $151.92 |
| Intact Protein ESI MS | $66.00 | $109.82 |
| LC/MS | $143.00 | $237.95 |
| LC/MS/MS – High sensitivity (triple quad) | $192.50 | $320.32 |
| LC/MS/MS – Normal sensitivity (triple quad) | $143.00 | $237.95 |
| MALDI MS | $66.00 | $109.82 |
| MALDI MS/MS | $77.00 | $128.13 |
| Offline HPLC use (8 hr) | $170.50 | $283.71 |
| Orbitrap LC/MS/MS – Long | $286.00 | $475.90 |
| Orbitrap LC/MS/MS – Short | $192.50 | $320.32 |
| Protein Concentration Assay | $110.00 | $183.04 |
| Solid-phase extraction | $24.20 | $40.27 |
| Solution Digest (trypsin/Lys-C) | $99.00 | $164.74 |
IRMS Rates:
| Product | Internal UW Rate | External Rate | |
| 18O (IRMS) | $31.82 | $48.68 | |
| 2H (IRMS) | $33.48 | $51.22 | |
| 2H and 18O via CRDS available for water samples only, please inquire |
|||
| 13C in CO2 | $12.07 | $18.47 | |
| 13C & 15N in Dry Matter* | $16.75 | $25.62 | |
| Consumables Fee | $220 | $336 | |
| Urinary Nitrogen | $22.93 | $35.08 | |
| Bomb Calorimetry | $36.33 | $55.58 | |
| Consulting (per hour) | $94 | $144 |